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Citations of IDT products

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9 Citations found

Brinkman EK, Kousholt AN, et al. (2018) Easy quantification of template-directed CRISPR/Cas9 editing. Nucleic Acids Res. doi: 10.1093/nar/gky164

This paper describes a rapid, cheap, and accessible analytic tool called TIDER (Tracking of Insertions, Deletions and Recombination events) that can be used to quantify incorporation frequency CRISPR-directed mutations. TIDER is derived from the widely used TIDE and the researchers here used the RNP CRISPR approach from IDT.

Sharma N, Hoshika S, et al. (2014) Recombinase-based isothermal amplification of nucleic acids with self-avoiding molecular recognition systems (SAMRS). Chembiochem, 15 : 2268-2274.

Researchers at the Foundation for Applied Molecular Evolution (Gainesville, FL, USA) use Ultramer DNA Oligos in experiments demonstrating the practical improvements that can be made to recombinase polymerase amplification (RPA), through addition of self-avoiding molecular recognition systems (SAMRS) to RPA-primers. 

Cartwright CP, Lembke BD, et al.. (2012) Development and validation of a semiquantitative, multitarget PCR Assay for diagnosis of bacterial vaginosis . J Clin Microbiol, 50 : 2321–2329.

Ultramer Oligonucleotides were used to construct calibration standards. Each Ultramer contained a target sequence from one of the transcripts under study. All qPCR assays included a set of 3 Ultramer calibrators.

Zielke K, Full F, et al.. (2012) The insulator protein CTCF binding sites in the orf73/LANA promoter region of herpesvirus saimiri are involved in conferring episomal stability in latently infected human T cells. J Virol, 86 : 1862–1873.

Ultramer Oligonucleotides were used for BAC mutagenesis via a two-step l–red mediated recombination strategy.

Flynn RA, Almada AE, Zamudio JR, Sharp PA. (2011) Antisense RNA polymerase II divergent transcripts are P-TEFb dependent and substrates for the RNA exosome. Proc Natl Acad Sci USA, 108 : 10460–10465.

Ultramer Oligonucleotides were used to generate standard curves for absolute quantification. Each Ultramer standard was designed to contain the 5′-end of a transcript under study and qPCR amplified using transcript-specific primers.

Balibar CJ, Hollis-Symynkywicz MF, Tao J. (2011) Pantethine rescues phosphopantothenoylcysteine synthetase and phosphopantothenoylcysteine decarboxylase deficiency in Escherichia coli but not in Pseudomonas aeruginosa. J Bacteriol, 193 : 3304–3312.

Ultramer oligonucleotides were used to construct a new vector that provides one-step incorporation of the PBAD promoter in front of any gene.

Ghiselli F, Milani L, Passamonti M. (2011) Strict sex-specific mtDNA segregation in the germ line of the DUI species Venerupis philippinarum (Bivalvia: Veneridae). Mol Biol Evol, 28 : 949–961.

Ultramer Oligonucleotides were used as standards due to their purity and quantification precision.

Incoronato M, Garofalo M, et al.. (2010) miR-212 increases tumor necrosis factor-related apoptosis-inducing ligand sensitivity in non-small cell lung cancer by targeting the antiapoptotic protein PED. Cancer Res, 70 : 3638–3646.

In situ detection of mature microRNAs via labeled extension of Ultramer templates is used to detect human miR-212 expression patterns.

Gibson DG. (2009) Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides. Nucleic Acids Res, 37 : 6984–6990.

Sets of hybridized, high-fidelity Ultramer Oligonucleotides were assembled into large constructs in a single step. Such constructs are available from IDT as gBlocks® Gene Fragments.