Choose your region, country/territory, and preferred language

22_DR_ProductOverview_HeaderIcon_DNARNA_180x180

ReadyMade™ Primers

HPLC purified, inventoried oligonucleotides for sample preparation, sequencing, and gene expression analysis of common genes.

Ordering

ReadyMade Primers include random hexamers, T7 promoter/terminator, M13 primers, 16S rRNA gene primers, and varieties of oligo dT that are available for same-day shipping.

  • Routine purity >90%
  • Identity confirmed via ESI-mass spectrometry*
  • Obtain 10 µg of dry product—enough for 100 sequencing reactions

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

ReadyMade™ Primers

10 µg of >90% pure primer as measured by CE. Identity confirmed by mass spec*.
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
Quantity Product Catalog # Sequence Unit Size Price Order Link
16S rRNA For 51-01-19-06 AGA GTT TGA TCC TGG CTC AG $11.50 USD Click here to order
16S rRNA Rev 51-01-19-07 ACG GCT ACC TTG TTA CGA CTT $11.50 USD Click here to order
3' RACE PCR 51-01-18-02 GGC CAC GCG TCG ACT AGT AC $11.50 USD Click here to order
Anchored Oligo dT (20) 51-01-15-08 TTT TTT TTT TTT TTT TTT TV $11.50 USD Click here to order
Anchored Oligo dT (22) 51-01-15-09 TTT TTT TTT TTT TTT TTT TTV N $11.50 USD Click here to order
BGH Reverse 51-01-02-08 TAG AAG GCA CAG TCG AGG $11.50 USD Click here to order
Bluescript KS 51-01-02-11 TCG AGG TCG ACG GTA TC $11.50 USD Click here to order
cDNA Cloning Primer 51-01-03-01 GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TV $11.50 USD Click here to order
EGFP-C 51-01-05-04 CAT GGT CCT GCT GGA GTT CGT G $11.50 USD Click here to order
EGFP-N 51-01-05-05 CGT CGC CGT CCA GCT CGA CCA G $11.50 USD Click here to order
GAPDH For 51-01-07-12 ACC ACA GTC CAT GCC ATC AC $11.50 USD Click here to order
GAPDH Rev 51-01-07-13 TCC ACC ACC CTG TTG CTG TA $11.50 USD Click here to order
M13 Forward (-20) 51-01-13-01 GTA AAA CGA CGG CCA GT $11.50 USD Click here to order
M13 Forward (-41) 51-01-13-02 CGC CAG GGT TTT CCC AGT CAC GAC $11.50 USD Click here to order
M13 Reverse (-27) 51-01-13-03 CAG GAA ACA GCT ATG AC $11.50 USD Click here to order
M13 Reverse (-48) 51-01-13-04 AGC GGA TAA CAA TTT CAC ACA GG $11.50 USD Click here to order
Neomycin For 51-01-14-01 CTT GGG TGG AGA GGC TAT TC $11.50 USD Click here to order
Neomycin Rev 51-01-14-02 AGG TGA GAT GAC AGG AGA TC $11.50 USD Click here to order
Oligo dT, (20) w/5' Phos 51-01-15-02 /5Phos/TTT TTT TTT TTT TTT TTT TT $11.50 USD Click here to order
Oligo dT, 15 mer 51-01-15-05 TTT TTT TTT TTT TTT $11.50 USD Click here to order
Oligo dT, 16 mer 51-01-15-06 TTT TTT TTT TTT TTT T $11.50 USD Click here to order
Oligo dT, 18 mer 51-01-15-07 TTT TTT TTT TTT TTT TTT $11.50 USD Click here to order
Oligo dT, 20 mer 51-01-15-01 TTT TTT TTT TTT TTT TTT TT $11.50 USD Click here to order
PCMV Forward 51-01-16-06 CGC AAA TGG GCG GTA GGC GTG $11.50 USD Click here to order
pET 3’ 51-01-16-07 CTA GTT ATT GCT CAG CGG $11.50 USD Click here to order
pET 5’ (T7) 51-01-16-08 TAA TAC GAC TCA CTA TAG G $11.50 USD Click here to order
pGEX 3’ 51-01-16-11 CCG GGA GCT GCA TGT GTC AGA GG $11.50 USD Click here to order
pGEX 5’ 51-01-16-12 GGG CTG GCA AGC CAC GTT TGG TG $11.50 USD Click here to order
Random Hexamer 51-01-18-01 NNN NNN $11.50 USD Click here to order
Random Hexamer w/Biotin 51-02-18-01 /5Biosg/NNN NNN $11.50 USD Click here to order
SP6 Promoter 51-01-19-05 TAC GAT TTA GGT GAC ACT ATA G $11.50 USD Click here to order
SP6 upstream 51-01-19-02 ATT TAG GTG ACA CTA TAG $11.50 USD Click here to order
T3 Promoter 51-01-20-03 AAT TAA CCC TCA CTA AAG GG $11.50 USD Click here to order
T7 Promoter 51-01-20-01 TAA TAC GAC TCA CTA TAG GG $11.50 USD Click here to order
T7 Terminator 51-01-20-02 GCT AGT TAT TGC TCA GCG G $11.50 USD Click here to order


Success!

Loading Page...

Product details

ReadyMade Primers are stocked oligonucleotides for sample preparation, PCR, sequencing, and gene expression analysis of common genes. Each primer contains 10 μg of HPLC-purified product. Identity is confirmed by mass spectrometry* and purity is established by capillary electrophoresis. Because these primers are inventoried, they can be shipped as soon as your order is received. Examples of ReadyMade Primers include random hexamers, T7 promoter/terminator, M13 primers, 16S rRNA primers, and varieties of Oligo dT. See the product list for our full catalog of ReadyMade Primers and their specific properties.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

PrimerSequence Tm (qPCR conditions)Spec Sheet (50nM Na+)Anhydrous MWpmol in
10 µg
16S rRNA For AGA GTT TGA TCC TGG CTC AG 61.354.36148.0 1626.7
16S rRNA Rev ACG GCT ACC TTG TTA CGA CTT 63.5 56.1 6372.2 1569.4
3' RACE PCR GGC CAC GCG TCG ACT AGT AC 66 60.1 6103.0 1638.5
Anchored Oligo dT (20) TTT TTT TTT TTT TTT TTT TV 48 37.5 6028.3 1658.7
Anchored Oligo dT (22) TTT TTT TTT TTT TTT TTT TTV N 51.5 41 6641.4 1505.7
BGH Reverse TAG AAG GCA CAG TCG AGG 60.2 53.6 5597.7 1786.4
Bluescript KS TCG AGG TCG ACG GTA TC 59.5 53.2 5226.4 1913.4
cDNA Cloning Primer GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TV 70.2 61.5 11584.9 863.1
EGFP-C CAT GGT CCT GCT GGA GTT CGT G 66.6 60.2 6773.4 1476.3
EGFP-N CGT CGC CGT CCA GCT CGA CCA G 71.7 66.4 6657.3 1502.1
GAPDH For ACC ACA GTC CAT GCC ATC AC 64.5 57.7 5991.0 1669.3
GAPDH Rev TCC ACC ACC CTG TTG CTG TA 65.4 58.6 6003.9 1665.7
M13 Forward (-20) GTA AAA CGA CGG CCA GT 59.4 52.6 5228.5 1912.6
M13 Forward (-41) CGC CAG GGT TTT CCC AGT CAC GAC 70.3 64.1 7289.8 1371.7
M13 Reverse (-27) CAG GAA ACA GCT ATG AC 54.2 47 5212.5 1918.3
M13 Reverse (-48) AGC GGA TAA CAA TTT CAC ACA GG 63.4 55.7 7065.7 1415.2
Neomycin For CTT GGG TGG AGA GGC TAT TC 61.6 54.9 6204.1 1612.0
Neomycin Rev AGG TGA GAT GAC AGG AGA TC 60.3 53.2 6255.1 1598.7
Oligo dT, 15mer TTT TTT TTT TTT TTT 38.6 28.4 4501.0 2221.7
Oligo dT, 16mer TTT TTT TTT TTT TTT T 40.9 30.64805.2 2081.0
Oligo dT, 18mer TTT TTT TTT TTT TTT TTT 44.8 34.3 5413.6 1847.3
Oligo dT, 20mer TTT TTT TTT TTT TTT TTT TT 48 37.3 6022.0 1660.6
Oligo dT, 20mer w/ 5' Phos /5Phos/TTT TTT TTT TTT TTT TTT TT 48 37.3 6101.9 1638.8
PCMV Forward CGC AAA TGG GCG GTA GGC GTG 69.8 63.9 6552.3 1526.2
pET 3' CTA GTT ATT GCT CAG CGG 57.1 50.2 5505.6 1816.2
pET 5' (T7) TAA TAC GAC TCA CTA TAG G 52.4 44.5 5795.8 1725.3
pGEX 3' CCG GGA GCT GCA TGT GTC AGA GG 70 64 7145.7 1399.3
pGEX 5' GGG CTG GCA AGC CAC GTT TGG TG 71.8 65.7 7136.6 1401.1
Random Hexamer NNN NNN <10 <10 1791.7 5581.3
Random Hexamer w/ Biotin /5Bio/NNN NNN <10 <10 2198.2 4549.3
SP6 Promoter TAC GAT TTA GGT GAC ACT ATA G 56.9 48.8 6773.5 1476.2
SP6 Upstream ATT TAG GTG ACA CTA TAG 50.2 42.1 5537.7 1805.8
T3 Promoter AAT TAA CCC TCA CTA AAG GG 57.1 49.3 6094.0 1640.9
T7 Promoter TAA TAC GAC TCA CTA TAG GG 55.2 47.5 6125.0 1632.5
T7 Terminator GCT AGT TAT TGC TCA GCG G 60.3 53.4 5834.8 1713.8
RUO22-1228_002