The xGen 16S Amplicon Panel v2 and the xGen ITS1 Amplicon Panel offer a reliable NGS workflow that provides coverage and NGS quality data on Illumina® sequencing platforms. These kits leverage multiplex PCR technology, enabling library
construction from DNA using tiled primer pairs to target V1-V9 variable rRNA regions and the ITS1 region, each with a single pool of multiplexed primer pairs.
These xGen Amplicon Panel kits utilize multiple overlapping amplicons in a single tube, using a rapid, 2-hour sequencing workflow to prepare ready-to-sequence libraries for research studies (Figure 1). The single pool multiplex PCR workflow generates
robust libraries, even from low-input quantities. The libraries may be quantified with conventional methods such as Qubit® (Thermo Fisher Scientific) or Bioanalyzer® (Agilent) and normalized by manual pooling or may be normalized
enzymatically with the included xGen Normalase™ reagents. (Note: using the Normalase reagent adds additional time to complete the workflow).
In addition, both the xGen 16S Amplicon Panel v2 and the xGen ITS1 Amplicon Panel facilitate NGS analysis of complex microbial communities (e.g., bacteria, archaea, fungi) using single primer pools that target the 16S rRNA gene (variable regions 1–9) and ITS1 region, respectively. The panels can be customized
with additional targets, including antibiotic resistance or virulence genes, allowing sub-genera level identification and functional analysis.