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Hybridization capture


Hybridization capture is a targeted next generation sequencing method that uses long, biotinylated oligonucleotide baits (probes) to hybridize to the regions of interest. It is particularly helpful when genotyping, rare variants, and exome sequencing.

Figure 1. Illustration of the precapture multiplexing method before NGS. Libraries are pooled at equal mass before target enrichment to conserve reagents while increasing workflow efficiency.
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xGen Exome Research Panel v2 white paper

Learn how our large-scale production platform, using PCR-free synthesis, provides a unique advantage over array-based platforms by delivering consistent exome panel performance over time.

Improving accuracy with unique molecular identifiers

For sequencing runs that require additional sequencing precision, adapters with unique molecular identifiers (UMIs) can be used with hybridization capture. UMIs are like indexes in that they facilitate identification of specific molecules within a sample. They can be used to remove duplicates generated during PCR that may create a bias. This is particularly helpful for providing more accurate quantification of true coverage in a region because of more accurate deduplication. UMIs can also be used for error correction to remove PCR and sequencing errors, which is particularly important for low-frequency variant. By removing errors, you can increase variant accuracy while significantly minimizing false positives.

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Combining NGS technologies for biomarker identification and confirmation research application note

IDT has developed 2 unique, high-performing targeted NGS approaches to maximize researchers’ ability to confidently identify and confirm biomarkers in germline and somatic mutations. Here, we describe the benefits and caveats of using either hybridization capture or amplicon sequencing to identify mutations in normal tissue, formalin-fixed, paraffin-embedded (FFPE) tumor, and cell-free DNA (cfDNA). 

Get started with hybridization capture

IDT offers a suite of pre-designed xGen hybridization capture panels for targeted next generation sequencing research. Choose from eight different panels including the Exome Hyb Panel v2, AML Cancer Hyb Panel, SARS-CoV-2 Hyb Panel, and the Inherited Diseases Panel. Don’t have what you’re looking for? No problem. IDT can also help you design custom hybridization capture probes for your specific research applications. Contact us today to learn more about getting started with hybridization capture.

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