Germline characterization with no concern for sample crosstalk
i.e., SNPs, indels, crude CNVs
SI, CDI, or UDI-UMI
Germline characterization where sample cross talk is important
i.e., translational research, inherited genetics, infectious disease
Low-level mutation detection, down to ~1% frequency
i.e., cancer, mosaicism, other diseases where somatic mutations are important
TruSeq™–Compatible Full-length Adapters
TruSeq™–Compatible Stubby Adapter
TruSeq™– or Nextera™–Compatible Indexing Primers
Forward and reverse primer mix
20 μM (10 μM each primer)
Suggested amount/reaction to use (may vary by application)
75 pmol in 5 μl
75 pmol in 5 μl
50 pmol (per primer) in 5 μl
IDT understands the needs of genomics projects are not one-size-fits-all, and provides custom adapters that you can tailor to your needs. Our scientists have created this Custom Adapter Configurator tool to guide you through selection of several options as part of the adapter design process.
Consecutive, starting with index #1 and ending with index #
Consecutive, starting with index # and ending with index #
If spreadsheet contains more than one worksheet, the worksheet with import data must be named "Sheet1"
File can contain no more than 2000 indexes
Spreadsheet should contain header columns in Row 1 and have the following values in the following spreadsheet cells:
B1: I5Name (not used)
C1: I5Barcode (not used)
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Please specify an Excel file.
To download sample Excel upload document, click here
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Note: We suggest to avoid using custom adapters (such as those through the Custom Adapter Configurator Tool) and off-the-shelf, stocked adapters together in the same project. Please contact us for further assistance.
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