xGen™ 56G Oncology Amplicon Panel

The xGen 56G Oncology Amplicon Panel v2 offers comprehensive and hotspot coverage of 56 oncology-related genes. The panel contains a 263-amplicon design to generate multiplex libraries compatible with Illumina® sequencing platforms, and 104 exonic and gender Sample_ID amplicons for gender identification for tracking tumor-normal pairs and samples in longitudinal studies.

xGen 56G Oncology Amplicon Panel
Amplicons263
Amplicon Size92–183 (Average 139 bp)
Number of Genes56
Genes List

ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, DDR2, DNMT3A, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, FOXL2, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MAP2K1, MET, MLH1, MPL, MSH6, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, TSC1, VHL

(Bold indicates whole CDS coverage)

Total Target23.7 kb
Input Recommended~10 ng DNA
Time Required2.5 hours from DNA-to-libraries
Limit of Detection>1%
Sample CompatibilityCell line, whole blood, cfDNA, FFPE
Multiplexing on MiSeq® v3 at 5000X depth (50 M PE reads)34 samples

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xGen 57G Pan-Cancer Amplicon Panel

The xGen 57G Pan-Cancer Amplicon Panel offers comprehensive and hotspot coverage of 57 oncology-related genes. The panel generates amplicons that cover over 16,000 COSMIC and 6,000 ClinVar mutations, as well as 104 exonic and gender markers for sample identification. In addition, the panel includes full exon coverage of TP53.

xGen Amplicon 57G Pan-Cancer Amplicon Panel
Amplicons286
Amplicon Size92–183 (Average 139 bp)
Number of Genes57
Genes List

ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, DDR2, DNMT3A, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, FOXL2, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MAP2K1, MET, MLH1, MPL, MSH6, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, TSC1, TSC2, VHL

(Bold indicates whole CDS coverage)

Total Target25.9 kb
Input Recommended~10 ng DNA
Time Required2.5 hours from DNA-to-libraries
Limit of Detection> 1%
Sample CompatibilityCell line, whole blood, cfDNA, FFPE
Multiplexing on MiSeq® v3 at 5000X depth (50 M PE reads)34 samples

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xGen TP53 Amplicon Panel

The xGen™ TP53 Panel offers comprehensive coverage of all coding regions of the TP53 gene, using a 21-amplicon design to generate multiplex libraries compatible with Illumina sequencing platforms and is compatible with short DNA fragments from both FFPE and cfDNA samples.

FeaturexGen TP53 Amplicon Panel
Amplicons21
Amplicon Size106–154 (Average 140 bp)
Number of Genes1
Genes ListTP53 (full CDS)
Total Target1.8 kb
Input Recommended~10 ng
Time Required2.5 hours from DNA-to-libraries
Limit of Detection>1%
Sample CompatibilityCell line, whole blood, cfDNA, FFPE
Multiplexing on MiSeq® v2 at 5000X Depth285 Samples

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xGen EGFR Pathway Amplicon Panel

The xGen EGFR Pathway Amplicon Panel generates 17 amplicons with an average size of 136 bp that cover hotspots in 4 key genes in the EGFR pathway─BRAF, KRAS, NRAS, and contiguous regions of EGFR.

FeaturexGen EGFR Pathway Amplicon Panel
Amplicons17
Amplicon Size107–155 (Average 136 bp)
Number of Genes4
Genes ListEGFR, BRAF, KRAS, NRAS
Total Target1.5 kb
Input Recommended~10 ng
Time Required2.5 hours from DNA-to-libraries
Limit of Detection>1%
Sample CompatibilityCell line, whole blood, cfDNA, FFPE
Multiplexing on MiSeq® v2 at 5000X Depth350

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xGen BRCA1 BRCA2 Amplicon Panel

The xGen BRCA1 BRCA2 Amplicon Panel generates 246 amplicons with an average size of 148 bp that covers the entire coding sequence of BRCA1 and BRCA2 genes.

FeaturexGen BCRA1/2 Amplicon Panel
Amplicons246
Amplicon Size107–208 (Average 146 bp)
Number of Genes2
Genes ListBRCA1, BRCA2
Total Target23.0 kb
Input Recommended~10 ng
Time Required2.5 hours from DNA-to-libraries
Limit of Detection> 1%
Sample CompatibilityFFPE, fresh frozen, genomic DNA
Multiplexing on MiSeq® v2 at 500X Depth (germline)243

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xGen BRCA1 BRCA2 PALB2 Amplicon Panel

The xGen BRCA1 BRCA2 PALB2 Amplicon Panel generates 302 amplicons with an average size of 149 bp that covers the entire coding sequence of BRCA1 and BRCA2 genes, as well as all coding exons and 5’ and 3’ UTR regions of PALB2.

FeaturexGen BRCA1 BRCA2 PALB2 Amplicon Panel
Amplicons302
Amplicon Size107–235 (Average 149 bp)
Number of Genes3
Genes ListBRCA1, BRCA2, PALB2
Total Target29.0 kb
Input Recommended~10 ng
Time Required2.5 hours from DNA-to-libraries
Limit of Detection> 1%
Sample CompatibilityFFPE, fresh frozen, genomic DNA
Multiplexing on MiSeq® v2 at 500X Depth (germline)198

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xGen CFTR Amplicon Panel

The xGen™ CFTR Amplicon Panel offers a comprehensive approach to investigate disease-relevant mutations and variants in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. This simple, rapid panel is used to research, discover, and confirm common and rare mutations in the CFTR gene. This unique panel utilizes 87 amplicons covering all exons, including 5’ and 3’ UTRs and regions of interest in introns 1, 12, 22, and 25. It also captures all ACMG‑recommended mutations, as well as offers a standardized solution to identify additional variants. The CFTR panel requires sample input as low as 10 ng per sample and is compatible with multiple sample types.

FeaturexGen CFTR Amplicon Panel
Amplicons87
Amplicon Size120–250 (Average 172 bp)
Number of Genes1
Genes ListCFTR (All exons including 5’ and 3’ UTRs, select intronic regions (1, 12, 22, and 25))
Total Target10.0 kb
Input Recommended~10 ng
Time Required2.5 hours from DNA-to-libraries
Limit of Detection> 1%
Sample CompatibilityFFPE, fresh frozen, genomic DNA
Multiplexing on MiSeq® v2 at 500X Depth (germline)384

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xGen Colorectal Amplicon Panel

xGen™ Colorectal Cancer Panel offers comprehensive and exon-level hotspot coverage of 16 clinically relevant colorectal genes. The panel covers over 11,000 COSMIC and 3,500 ClinVar mutations and generates targeted libraries compatible with Illumina® sequencing platforms. In addition, the panel includes full exon coverage of TP53 and enables validated CNV detection in the ERBB2 gene. Our customization allows you to add focused content to achieve your most optimal design. Either combine pre-validated content such as our Lynch Syndrome content or add your own targets.

FeaturexGen Colorectal Amplicon Panel
Amplicons186
Amplicon Size103–183 (Average 143 bp)
Number of Genes16
Genes List

AKT1, APC, BRAF, ERBB2, ERBB4, KIT, KRAS, NOTCH1, NRAS, PDGFRA, PIK3CA, POLE, PTEN, SMAD4, STK11, TP53

(Bold indicates whole CDS coverage)

Total Target17.0 kb
Input Recommended~10 ng
Time Required2.5 hours from DNA-to-libraries
Limit of Detection>1%
Sample CompatibilityCell line, whole blood, cfDNA, FFPE
Multiplexing on MiSeq® v2 at 5000X Depth 32

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xGen Lung Amplicon Panel

The xGen Lung Amplicon Panel offers comprehensive and exon-level hotspot coverage of 17 key genes for studying both small cell lung cancer (SCLC) and non-small lung cancer (NSCLC). The panel covers over 11,000 COSMIC and 2,500 ClinVar mutations and generates targeted libraries compatible with Illumina® sequencing platforms.

FeaturexGen Lung Amplicon Panel
Amplicons208
Amplicon Size106–165 (Average 142 bp)
Number of Genes17
Genes List

AKT1, ALK, ARAF, BRAF, EGFR, ERBB2, ERBB4, FGFR1, FGFR2, FGFR3, KRAS, MAP2K1, MET, NRAS, PIK3CA, PTEN, TP53

(Bold indicates whole CDS coverage)

Total Target18.0 kb
Input Recommended~10 ng
Time Required2.5 hours from DNA-to-libraries
Limit of Detection>1%
Sample CompatibilityCell line, whole blood, cfDNA, FFPE
Multiplexing on MiSeq® v2 at 5000X Depth28

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xGen Lynch Syndrome Amplicon Panel

A multigene panel to identify mutations in DNA Mismatch Repair (MMR) genes with comprehensive and exon-level hotspot coverage of 4 clinically-relevant genes: MLH1, MSH2, MSH6, and PMS2. And with sensitivity down to 1% allele frequency to identify both germline and somatic mutations in the same panel.

FeaturexGen Lynch Syndrome Amplicon Panel
Amplicons180
Amplicon Size116–150 (Average 139 bp)
Number of Genes4
Genes ListMLH1, MSH2, MSH6, PMS2
Total Target15 kb
Input Recommended~10 ng
Time Required2.5 hours from DNA-to-libraries
Limit of Detection>1%
Sample CompatibilityCell line, whole blood, cfDNA, FFPE
Multiplexing on MiSeq® v2 at 5000X Depth33

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xGen Myeloid Amplicon Panel

The xGen Myeloid Amplicon Panel offers comprehensive and exon-level hotspot coverage of 23 myeloid disease related genes. The panel covers over 12,000 COSMIC and 1,900 ClinVar mutations and generates targeted libraries compatible with Illumina® sequencing platforms.

FeaturexGen Myeloid Amplicon Panel
Amplicons478
Amplicon Size106–199 (Average 142 bp)
Number of Genes23
Genes List

ASXL1, CALR, CEBPA, CSF3R, DNMT3A, EZH2, FLT3, HRAS, IDH1, IDH2, JAK2, JAK3, KDM6A, KIT, MPL, NPM1, RUNX1, SETBP1, SF3B1, SRSF2, TET2, TP53, U2AF1

(Bold indicates whole CDS coverage)

Total Target42.0 kb
Input Recommended~10 ng
Time Required2.5 hours from DNA-to-libraries
Limit of Detection>1%
Sample CompatibilityCell line, whole blood, gDNA, FFPE
Multiplexing on MiSeq® v2 at 5000X Depth12

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