Cost effective genotyping assays for low to high throughput screening
PACE (PCR Allele Competitive Extension) SNP genotyping assays are well-suited for projects that require a reliable, cost-effective approach for SNP and indel detection. PACE is especially suitable for high volume screening projects, such as plant breeding research. IDT and 3CR Bioscience (UK) have partnered to provide optimally designed genotyping assays with a high performance master mix, all provided in a short delivery time and at the lowest overall cost in the industry. Learn more about our partnership and how PACE technology works.
Contact our experts to discuss whether this technology is right for your genotyping project. Contact us »
Order the PACE Genotyping Master Mix from 3CR by going to https://3crbio.com/products/information/.
The PACE (PCR Allele Competitive Extension) SNP genotyping system is a fluorescent, endpoint genotyping technology that uses competitive allele-specific PCR. It makes use of a universal PCR master mix (PACE Genotyping Master Mix) with allele-specific primers and a common reverse primer for bi-allelic genotyping. The PACE reaction is specific and produces fluorescent signal that is easy to detect on fluorescent plate readers and real time PCR systems.
The PACE genotyping method and industry-leading KASP system are both easy to use, and the 2 methods produce very similar quality data, as shown in Figure 1. However, the PACE Master Mix is a better use of research funds, especially when using larger pack sizes.
Figure 1. The PACE genotyping system provides improved, cost-effective genotyping compared to the leading industry method, KASP. Genotyping was performed on purified corn DNA samples. Distinct genotyping assays (4) were selected to assess sequence variability that can affect genotyping outcome. (A) Normal GC/AT content (48% GC). (B) Slightly GC-rich content (68%), (C) AT-rich content (28% GC), and (D) Slighly AT-rich content (40% GC). master mixes for the PACE and KASP methods were used for genotyping each of the 4 assays (identical primers and samples), in 4 µL reactions, following each manufacturer's protocol. Assay results were analyzed as cluster plots. Genotyping clusters from the PACE reactions were as well or better defined than the KASP reactions. In addition, the clusters of the PACE reactions were optimal at a fixed number of PCR cycles, whereas with KASP reactions, different cycle numbers were needed.