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xGen™ Oncology & Inherited Diseases Amplicon Panels
Compatible with FFPE and cell-free DNA samples
Confident variant identification as low as 1% allele frequency facilitates research on tumor growth and identification of driver mutations in a fast, easy, single-tube workflow.
xGen NGS—made for oncology and inherited disease research.
Ordering
| xGen Amplicon Panel | Specifications link | BED Files link/download | |
|---|---|---|---|
| Oncology Research Panels | xGen 56G Oncology Amplicon Panel | Specifications | Panel Targets |
| xGen 57G Pan-Cancer Amplicon Panel | Specifications | Panel Targets | |
| xGen TP53 Amplicon Panel | Specifications | Panel Targets | |
| xGen EGFR Pathway Amplicon Panel | Specifications | Panel Targets | |
| xGen BRCA1/BRCA2 Amplicon Panel | Specifications | Panel Targets | |
| xGen BRCA1/BRCA2 PALB2 Amplicon Panel | Specifications | Panel Targets | |
| xGen Colorectal Amplicon Panel | Specifications | Panel Targets | |
| xGen Lung Amplicon Panel | Specifications | Panel Targets | |
| xGen Lynch Syndrome Amplicon Panel | Specifications | Panel Targets | |
| xGen Myeloid Amplicon Panel | Specifications | Panel Targets | |
| Inherited Disease Research Panel | xGen CFTR Amplicon Panel | Specifications | Panel Targets |
- Compatible with Illumina® sequencing platforms
- Designed for research on germline and somatic variant identification, or targeted sequencing
- Offers overlapping amplicons in a fast, easy, single-tube workflow
xGen™ Amplicon Core Kit
Targeted amplicon library prep kit for contiguous coverage (overlapping amplicons), comprises multiplexed PCR, indexing PCR, and Normalase™ reagents (target-specific panel and indexing primers sold separately).
| Quantity | Product | Catalog # | Price | ||
|---|---|---|---|---|---|
| xGen™ Amplicon Core 96rxn | 10009827 | $2,945.00 USD |
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xGen™ Oncology/Inherited Disease Amplicon Panels
Oncology and infectious disease panels that utilize multiple overlapping amplicons in a single tube; comprises a premixed target-specific primer pool.
| Quantity | Product | Catalog # | Price | ||
|---|---|---|---|---|---|
| xGen™ 56G Onco Amplicon Panel v2 96rxn | 10009838 | $7,045.00 USD |
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| xGen™ 57G PanCancer Amplicon Panel 96rxn | 10009837 | $7,045.00 USD |
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| xGen™ BRCA1/2 Amplicon Panel 96rxn | 10009840 | $329.00 USD |
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| xGen™ BRCA1/2 PALB2 Amplicon Panel 96rxn | 10009841 | $7,045.00 USD |
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| xGen™ CFTR Amplicon Panel 96rxn | 10009844 | $1,390.00 USD |
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| xGen™ Colorectal Amplicon Panel 96rxn | 10009834 | $5,280.00 USD |
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| xGen™ EGFR Pathway Amplicon Panel 96rxn | 10009839 | $1,390.00 USD |
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| xGen™ Lung Amplicon Panel 96rxn | 10009833 | $5,280.00 USD |
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| xGen™ Lynch Syndrome Amplicon Panel 96rxn | 10009835 | $5,280.00 USD |
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| xGen™ Myeloid Amplicon Panel 96rxn | 10009836 | $6,605.00 USD |
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| xGen™ TP53 Amplicon Panel 96rxn | 10009842 | $1,390.00 USD |
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xGen™ Amplicon UDI Primers
Premixed, unique dual index primers designed for Normalase™ workflow. Individual plates of 96 indexes or sets of 384 indexes are available. Or choose all four sets for a total of 1536 index pairs. All plates are single-use to minimize the risk of contamination by multiple handling events.
| Quantity | Product | Catalog # | Price | ||
|---|---|---|---|---|---|
| xGen™ Amplicon UDI Primers Plate 1 | 10009847 | $664.00 USD |
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| xGen™ Amplicon UDI Primers Plate 2 | 10009848 | $664.00 USD |
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| xGen™ Amplicon UDI Primers Plate 3 | 10009849 | $664.00 USD |
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| xGen™ Amplicon UDI Primers Plate 4 | 10009850 | $664.00 USD |
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| xGen™ Amplicon UDI Primers Set 1 | 10009846 | $2,670.00 USD |
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| xGen™ Amplicon UDI Primers Set 2 | 10009851 | $2,670.00 USD |
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| xGen™ Amplicon UDI Primers Set 3 | 10009852 | $2,670.00 USD |
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| xGen™ Amplicon UDI Primers Set 4 | 10009853 | $2,670.00 USD |
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Figure 1. Plate combinations for 1536 UDI primer pairs. Each 96-well plate is sold in a set of four plates. For 1536 UDI primers, order Set 1, 2, 3, and 4.
For plate layouts of each of the 16 UDI plates offered see the xGen™ Normalase™ Indexing Primers quick reference guide.
xGen™ PEG NaCl Buffer
Buffer used during ''with bead'' purification steps when DNA is eluted into the next reaction mixture and the beads are reused. Supplied with the xGen Amplicon Core Kit but also sold separately if additional buffer is needed.
| Quantity | Product | Catalog # | Price | ||
|---|---|---|---|---|---|
| PEG NaCl Buffer | 10009854 | $27.00 USD |
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xGen™ Amplicon CDI Primers
Combinatorial dual index primers designed for Normalase workflow. Supports up to 96 combinations of i7-i5 indexes.
| Quantity | Product | Catalog # | Price | ||
|---|---|---|---|---|---|
| xGen™ Amplicon CDI Primers 96rxn | 10009845 | $756.00 USD |
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Request a consultation
Want to learn more about our xGen™ Amplicon Panels—developed with super amplicon technology—and how to customize your panel or spike-in genes in our predesigned panels? Your time is valuable—we’ll prioritize your inquiry and be in touch to discuss it ASAP.
Request a consultationProduct details
xGen Amplicon Panels for oncology and inherited diseases research utilize multiple overlapping amplicons in a single tube, using a rapid, 2.5-hour workflow to prepare ready-to-sequence libraries in research studies (Figure 2). The PCR1+PCR2 workflow generates NGS libraries for identifying genetic changes in the genes associated with a variety of tissues and inherited diseases (Table 1). The libraries may be quantified with conventional methods such as Qubit® (Thermo Fisher Scientific) or Agilent Bioanalyzer® and normalized by manual pooling or normalized enzymatically with the included xGen Normalase™ reagents.
Figure 2. xGen Amplicon Panels have a single tube workflow that is done in as little as 2.5 hours. Creating an NGS library starts with multiplex PCR. Your custom or predesigned panel is combined with the DNA
sample to amplify the targets of interest. The samples are then amplified with indexing primers to create a functional dual indexed library. As an optional step, the xGen Normalase reagent can be used after pooling multiple libraries to ensure each
is equally represented in the final sample for the flowcell.
Table 1. List of xGen Oncology & Inherited Diseases Amplicon Panels
| Panel | Number of Genes | Genes |
|---|---|---|
| xGen 56G Oncology Amplicon Panel v2, 96 rxn | 56 | ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, DDR2, DNMT3A, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, FOXL2, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MAP2K1, MET, MLH1, MPL, MSH6, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, TSC1, VHL |
| xGen 57G Pan-Cancer Amplicon Panel, 96 rxn | 57 | ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, DDR2, DNMT3A, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, FOXL2, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MAP2K1, MET, MLH1, MPL, MSH6, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, TSC1, TSC2, VHL |
| xGen BRCA1 BRCA2 Amplicon Panel, 96 rxn | 2 | BRCA1, BRCA2 |
| xGen BRCA1 BRCA2 PALB2 Amplicon Panel, 96 rxn | 3 | BRCA1, BRCA2, PALB2 |
| xGen CFTR Amplicon Panel, 96 rxn | 1 | CFTR (All exons including 5’ and 3’ UTRs, select intronic regions (1, 12, 22, and 25)) |
| xGen EGFR Pathway Amplicon Panel, 96 rxn | 4 | EGFR, BRAF, KRAS, NRAS |
| xGen Colorectal Amplicon Panel, 96 rxn | 16 | AKT1, APC, BRAF, ERBB2, ERBB4, KIT, KRAS, NOTCH1, NRAS, PDGFRA, PIK3CA, POLE, PTEN, SMAD4, STK11, TP53 |
| xGen TP53 Amplicon Panel, 96 rxn | 1 | TP53 |
| xGen Lung Amplicon Panel, 96 rxn | 17 | AKT1, ALK, ARAF, BRAF, EGFR, ERBB2, ERBB4, FGFR1, FGFR2, FGFR3, KRAS, MAP2K1, MET, NRAS, PIK3CA, PTEN, TP53 |
| xGen Lynch Syndrome Amplicon Panel, 96 rxn | 4 | MLH1, MSH2, MSH6, PMS2 |
| xGen Myeloid Amplicon Panel, 96 rxn | 23 | ASXL1, CALR, CEBPA, CSF3R, DNMT3A, EZH2, FLT3, HRAS, IDH1, IDH2, JAK2, JAK3, KDM6A, KIT, MPL, NPM1, RUNX1, SETBP1, SF3B1, SRSF2, TET2, TP53, U2AF1 |
Bold indicates whole CDS coverage
Resources
RUO21-0279_003.1