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Affinity Plus Probes

Items:
 
5' Dye 5' Code 3' Quencher 3' Code
5' 6-FAM /56-FAM/ 3' Iowa Black™ FQ /3IABkFQ/ Other
5' Cy®3 /5Cy3/ 3' Iowa Black™ RQ-Sp /3IAbRQSp/ Other
5' Cy®5 /5Cy5/ 3' Iowa Black™ RQ-Sp /3IAbRQSp/ Other
5' HEX /5HEX/ 3' Iowa Black™ FQ /3IABkFQ/ Other
5' SUN™ /5SUN/ 3' Iowa Black™ FQ /3IABkFQ/ Other
5' Yakima Yellow® /5YakYel/ 3' Iowa Black™ FQ /3IABkFQ/ Other
Show Other 5' Dyes Hide Other 5' Dyes
(5' → 3')
Base Notations
  • Standard Bases, (A, C, G, T) are not case sensitive.
  • Mixed Bases, expressed in UPPERCASE.
  • RNA, expressed as 'r_', for example rA, rU.
  • Base notation for Affinity Plus (locked nucleic acids) = +A, +G, +C, +T
  • 2' O-methyl RNA bases are entered as 'm_'.
  • Phosphorothioated DNA bases are entered as '_*'
  • Phosphorothioated RNA bases are entered as 'r_*'.
  • Phosphorothioated 2'-O-methyl RNA bases are entered as 'm_*'.
Standard
A,G C,G A,C,G
C,T A,T A,G,T
A,C A,C,T A,C,G,T
G,T C,G,T
Select a IUB symbol to insert it at the end of the current sequence.
Custom
%A %C %G %T
0/100
Enter desired percentage for each base. Click 'Apply' to append to end of sequence.
# Bases: 0 Base notation for Affinity Plus bases = +A, +G, +C, +T (available on single-quenched probes only).

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