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The lac operon, explained
The lac operon was first described in Escherichia coli in the 1960s and has led to important discoveries about gene regulation and how cells respond to changes in their environment. This DECODED provides an overview of the lac operon and explains how it has transformed the way researchers generate mutants.
- When deoxythymine (dT) is required for modification attachment
- Calculating melting temperature (Tm)
- Calculations: Converting from nanograms to copy number
- Which type of oligo purification should I choose?
- Scale and yield—Are they the same?
- Oligo quantification—Getting it right
- Alternative dyes for FISH, FRET, and qPCR
- Ordering modified oligonucleotides
- G repeats—Structural challenges for oligo design
- Calculators for resuspension and dilution of oligonucleotides
- Which biotin modification to use?
- Annealing oligos
- Determining the physical characteristics of your oligos
- Deoxyinosine: Don’t call me a universal base!
- Oligonucleotide modifications: Choosing the right mod for your needs
- The importance of melting temperature in molecular biology applications
- Oligo modification—Post-synthesis conjugation explained
- Fluorescein—Painting rivers green!
- Understanding mass spectrometry of oligonucleotides
- Running agarose and polyacrylamide gels
- Unraveling RNA—The importance of a 2' hydroxyl
DECODED article categories:
RUO23-1916_001